Flow Cytometry

Flow cytometry facilitates quantitative, multiparameter analysis of cell populations based on the expression of cell surface and/or intracellular molecules. R&D Systems offers a wide range of biotin-, fluorescein-, phycoerythrin-, PerCP-, AlexaFluor® 488-, or allophycocyanin-conjugated monoclonal antibodies specifically designed to monitor protein expression by flow cytometry. Also available are Fluorokine® kits; labeled cytokines that can be used to monitor the presence of cytokine receptors.

• Cell Surface Staining Antibodies for Flow Cytometry
  A rapid and simple method to immunophenotype cell populations.
• Fluorokine Receptor Detection Kits
  Biotinylated ligands for estimating receptor densities.
• Intracellular Staining Antibodies for Flow Cytometry
  Intracellular staining reagents offer a rapid and simple method to identify cells that secrete selected cytokines (e.g. Th1 vs. Th2). A variety of fluorochrome-labeled isotype controls, conjugated with the same fluorochrome to protein ratio, are available to aid in assessing background staining.
• Multi-Color Flow Cytometry Kits
  Kits to simultaneously label cells with up to four antibodies conjugated to four different fluorochromes.
• Flow Cytometry Supplemental Reagents
  Antibody isotype controls and supplemental staining reagents for flow cytometry.

AlexaFluor is a registered trademark of Life Technologies.

Flow Cytometry & Cell Selection/Detection

R&D Systems offers a choice of products for cell selection including our cell enrichment columns that use affinity negative selection and MagCellect‚Ñ¢ Cell Selection kits and PlusCellect‚Ñ¢ Cell Selection kits that utilize ferrofluids (magnetic particles) for magnetic separation. Our cell detection tools include flow cytometry kits for the assessment of receptor density and multi-color kits which can simultaneously measure up to four different target molecules.

• PlusCellect Cell Selection Kits
  Complete kits for selection and detection of cell populations
• MagCellect Cell Selection Kits
  Complete kits for selection of cell populations
• Flow Cytometry Antibodies and Kits
  A wide selection of antibodies, labeled ligands for assessing receptor density, and multi-color kits for simultaneously measuring the expression of up to four different molecules.
• Cell Enrichment Column Kits
• T Cell Enrichment Columns
  Kits for enrichment of different T cell populations by negative selection
• Supplemental Cell Selection Reagents & MagCellect Cell Selection Magnet
  Additional reagents for cell selection and detection experiments